Bio - Technology
Enzyme that transcribes DNA from RNA is called
A) Reverse transcriptase
B) DNA polymerase
C) Taq polymerase
D) RNA polymerase
For what purpose is the polymerase chain reaction used?
A) To cut DNA
B) To join DNA sequences
C) To amplify DNA sequences
D) To clone cells
Which of the following procedures would produce RFLPs?
A) Incbating DNA nucleotides with DNA polymerase
B) Incubating a mixture of single stranded DNA from two closely related species
C) Incubating DNA with restriction enzyme
D) None of these
Restriction enzymes are molecular scissors used in genetic engineering to cut up DNA. In nature where restriction enzymes found and what is their natural role
A) In viruses; to splice host cell DNA
B) In bacteria; to defend against viral invasion
C) In yeast cell; to defend against foreign DNA
D) In viruses; to defend against mutation
A method used to detect a particular DNA sequence within a mixture of many DNA fragments is
A) DNA sequencing
B) DNA finger printing
C) Colony hybridization
D) Southern blotting
When bacteria are genetically engineered to produce human insulin; the enzymes used in the procedure are:
1) Ligase
2) Restriction endonuclease
3) Reverse transcriptase.
In which order are the enzymes used?
A) 1-3-2
B) 2-3-1
C) 2-1-3
D) 3-2-1
Restriction fragment length polymorphism (RFLPs)
A) Are achieved by using restriction enzymes
B) Identify individuals genetically
C) Are the basis for DNA finger - printing
D) All the above are correct
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